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two pure substances. Basic techniques useful for the separation of mixtures include decantation, distillation, filtration, evaporation, and extraction. 5 Separation of Substances. Grain is separated from stalks, while harvesting. Milk or curd is churned to separate the butter (Fig. 5.2). As we. Deal with the separation of mixtures to enhance purity of substances Numerous techniques have been developed to separate mixtures to study components.Separating the solute from the solvent in a solution is usually much more difficult than separating undissolved substances from a liquid. Filtration won't work Mixtures can be separated into their di erent pure substance components based on the physical properties of the components. Pure substances have unique physical A physical processing which a particular mixture (a suspension of a participate in a liquid solution) is separated into a solid and a liquid. Separating funnel. When two or more substances, that do not react chemically, are blended together, the result is a mixture in which each component retains its individual Mixtures can be physically separated by using methods that use differences in physical properties to separate the components of the mixture, such as evaporation