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Works with a range of automated, semi-automated and manual extraction kits Real Time Instruments. Applied Biosystems 7500/7500 Fast, ViiA 7 (Thermo Fisher Scientific). Rotor-Gene 3000/6000/Rotor-Gene Q (Qiagen) CFX96 (Bio-Rad) LightCycler 480 (Roche). we also supply the required colour compensation kit. Rotor-Gene™ 3000 Rotor-Gene ™ 6000 Rotor-Gene™ Q Roche Lightcycler®96 Lightcycler®480 Lightcycler®Nano Takara Thermal Cycler Dice® Techne PrimeQ Quantica® Thermo Piko Real™ SensiFAST Selection Table. 5 Table 1 Applications for SensiFAST One‑Step kits Gene expression analysis Microarray validation Viral quantitation Pathogen detection Biomarker discovery and validation Gene Corbett Rotor-Gene™ 3000 and 6000 Real-Time Rotary Analyzer; Eppendorf Mastercycler® ep realplex Real-Time PCR System ; Roche LightCycler® 480 Real-Time PCR System; Stratagene Mx3000P® and Mx3005P® Real-Time PCR Systems; Stratagene Mx4000® Multiplex Quantitative PCR System; Protocols. Complete Protocol. GoTaq® qPCR Master Mix Technical Manual PDF (442 KB) - English. GoTaq® 1-Step RT Qiagen Rotor-Gene™ Eppendorf Mastercycler Cepheid SmartCycler Antylia Scientific Eco 48 Low ROX Biorad iCycler, MyiQ, MiQ 2, iQ 5, CFX96, CFX384, Chromo4, MJOptiocon, Opticon 2, MiniOpticon Cepheid SmartCycler Eppendorf Mastercycler Illumina Eco Real-Time PCR System Qiagen Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000 Roche LightCycler 480 Description. SsoAdvanced Universal SYBR Green Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. Diagnostic Sensitivity. 92.86% (CI 95%: 64.17% - 99.63%) Linear Range. 10 10 - 10 2.5 IU/ml with precision of ± 0.5 log. Dynamic Range. 10 10 - 122.594 cp/ml (using manual extraction GeneProof PathogenFree DNA Isolation Kit) 10 10 - 165.237 cp/ml (using automatic extraction croBEE NA16 Nucleic Acid Extraction Kit) Reporting Units. column. Centrifuge for 2 min. at 3 ,000 × g in a swinging buck et rotor. Discard the flow -through and place the column back into the same collection tube. 13 Repeat the column wash with Wash Solution II (step 1 2). 14 Disca rd the collection tube containing the flow -through. 15 Transfer the column into a fresh 15 mL collection tube (provided). Roto-Gene ® 3000/6000 Rotor-Gene ® Q 5/6 plex Platfom CFX96™ Real-Time System: Downloads. Manual RealStar ® GPR PCR Master 1.0. Product Information Sheet (PIS) pdf. File size: 172.43 KB. Document ID: PIS-8001000-EN-02: Material Safty Data Sheet (MSDS) pdf. File size: 243.46 KB. Document ID: SDS-80010-US-en-01 : Order. Get the product you need. Contact sales. Find your local sales rep or The GeneJET Endo-free Plasmid Maxiprep purification protocol outlined in this manual is optimized for use with bacterial cultures grown in Luria-Bertani (LB) medium to a cell density of approximately 2-3 (OD 600 = 2-3). The use of rich growth media is not recommended. Bacterial culture growth Qiagen: Rotor-Gene ® Q, Rotor-Gene 3000, Rotor-Gene® 6000 Eppendorf: Masterc