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This learning center is your guide to the theory and implementation of the qPCR technique as it is used in gene expression analysis, beginning with RNA Reagent Comparison Guide for Real-Time PCR. Visit us on the Web at bio-rad.com. 4. Reagent and Assay Comparison Matrix. Preparation of. Samples and. Choose from Bio-Rad's simple to sophisticated high-throughput qPCR systems CFX Real-Time PCR Detection Systems are powerful and flexible instruments,This guidebook comes with an easy-to-review real-time PCR quick guide to use at the bench. The quick guide covers the fundamentals of qPCR, design, optimization It includes guidelines for designing the best real-time PCR assay for your experiments and explains how real-time PCR data are used in various applications. Always use dedicated pipets for qPCR. 4. Use aerosol-resistant filter tips. 5. Use PCR-grade water. 6. Clean bench with 10% bleach, not ethanol! Real-time PCR can be used for both qualitative and quantitative analysis; choosing the best method for your Real-time qPCR Workflow Selection Guide. Perform one-step multiplex RT-qPCR with minimal cross-reactivity using Reliance One-Step Multiplex Supermix and. PrimePCR Probe Assays expertly designed for Learn to design, optimize, and validate real-time PCR assays. Primer and probe design, target selection, gradient, melt curve, and multiplexing for qPCR